Antioxidant Strategies 抗氧化方案

Antioxidant therapies have been studied extensively in the ART setting. Oral antioxidants are already employed in improving fertilization and pregnancy rates.

抗氧化剂疗法已经在辅助生殖领域得到了广泛的研究。口服抗氧化剂可以用于提高受精率和妊娠率。

In a study by Tamura et al. (1) 18 patients undergoing IVF-ET were treated within an oral supplement of 3mg melatonin, 600mg vitamin E, or a combination of both antioxidants once daily. Intracellular concentrations of 8-hydroxy-2-deoxyguanosine were significantly reduced after antioxidant treatment.

在Tamura等人的研究中(数据参考见1)，18例病人在试管治疗期间，接受口服补充3mg褪黑素、600mg维生素E或者每天一次两种抗氧化剂的组合。抗氧化剂口服治疗后，细胞内8-羟基-2-脱氧鸟苷的浓度显著降低。

The second part of the experiment involved 115 patients who had previously failed to get pregnant after one IVF-ET treatment cycle. Out of these women, 56 were treated with melatonin (3mg) daily, while the remaining 59 patients received no antioxidant supplementation. Fertilization rates increased significantly from the previous IVF-ET cycle within the melatonin treatment group, but no significance was observed when compared to the control group. The study concluded that melatonin improved oocyte and embryo quality and marginally increased fertilization rates.

二期实验受试者有115名先前试管移植失败的病人，其中56名每天服用3mg的褪黑素，剩余59名病人没有补充抗氧化剂。结果显示，褪黑素治疗组的受精率相比以往周期显著增加，而与对照组相比无显著性差异。这项研究表明，褪黑素改善了卵母细胞和胚胎质量，并略微提高了受精率。

Coenzyme Q10, also known as ubiquinone, is a substance mostly produced in the mitochondria of cells. Lewin et al. (2) performed a study on 22 patients, 16 of which were diagnosed as asthenozoospermic. Supplementation with 50 microM CoQ10 resulted in a significant increase in motile sperm from the subgroup of asthenozoospermic men. No significant increase in motility was seen in normozoospermic men. However, after daily oral treatment with 60mg/day CoQ10, for a mean of 103 days, a significant improvement in fertilization rate was observed. The authors conclude that ubiquinone supplementation is effective in selected patients.

辅酶Q10，也称为泛醌，是一种主要在细胞线粒体中产生的物质。Lewin等人(数据参考见2)对22例病人进行了研究，其中16例被诊断为弱精症。弱精症男性小组补充50μM的辅酶Q10后精子活力显著增加，正常男性精子活力无显著增加。然而，在每天口服60mg辅酶Q10治疗103天后，受精率得到显著改善。作者得出结论，泛醌补充剂对特定病人有效。

References 数据参考：

1. Tamura H, Takasaki A, Miwa I et al (2008) Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J Pineal Res 44(3):280-287

2. Lewin A, Lavon H(1997) The effect of coenzyme Q10 on sperm motility and function. Mol Aspects Med 18(Suppl):S213-S219